Table 3.
Analysis of IRC production with the colorimetric method, biotest, and liquid chromatography-tandem mass spectrometry determination of IAA from all IRCs. Colorimetric method—µg∙mL−1 all IRCs, Biotest -, na—not analyzed, (+)—significant result of the biotest, (-)—not significant result of the biotest. LC-MS/MS method—µg∙mL−1 IAA from all IRCs, nd—not detected. Data are presented as the mean (n = 3).
| Strain Name | Methods | Results | ||||||
|---|---|---|---|---|---|---|---|---|
| Time of Cultivation (h) | ||||||||
| 24 | 48 | 72 | 96 | 120 | 144 | 168 | ||
| HLA 1-7 | LC-MS/MS | 1.836 | na | na | na | 1.810 | 1.776 | na |
| Colorimetric method | 14.220 | 12.673 | 7.970 | 7.256 | 7.286 | nd | nd | |
| Biotest | - | - | - | - | - | na | na | |
| HLA 2-7 | LC-MS/MS | 0.344 | na | 0.702 | 0.668 | na | na | na |
| Colorimetric method | 10.708 | 21.839 | 7.792 | nd | nd | nd | nd | |
| Biotest | - | - | - | - | - | na | na | |
| HLB 1-3 | LC-MS/MS | 0.519 | na | 0.720 | 0.686 | na | na | na |
| Colorimetric method | 13.536 | 20.9762 | 10.232 | nd | nd | nd | nd | |
| HLB 2-6 | LC-MS/MS | 1.716 | 3.958 | 4.643 | na | na | na | na |
| Colorimetric method | 12.077 | 10.024 | nd | nd | nd | nd | nd | |
| Biotest | - | - | - | - | na | na | na | |
| K 1-4 | LC-MS/MS | 0.718 | na | na | 0.731 | 0.711 | na | na |
| Colorimetric method | nd | 12.375 | 11.899 | 11.214 | nd | nd | nd | |
| KC 1-4 | LC-MS/MS | 2.789 | na | na | na | na | na | 6.649 |
| Colorimetric method | 33.298 | 25.977 | 24.607 | 25.500 | 22.851 | 24.012 | 18.446 | |
| Biotest | + | + | + | + | na | na | na | |
| PC 2-5 | LC-MS/MS | 11.770 | na | na | na | 7.178 | 7.181 | na |
| Colorimetric method | 28.327 | 20.917 | 15.054 | 18.506 | 13.625 | nd | nd | |
| Biotest | + | + | + | + | + | na | na | |
| RL 2-2 | LC-MS/MS | na | 0.357 | na | na | 0.301 | 0.360 | na |
| Colorimetric method | nd | nd | 18.536 | 13.893 | 13.506 | nd | nd | |
| RP 2-4 | LC-MS/MS | na | na | 0.650 | 0.702 | 0.500 | na | na |
| Colorimetric method | nd | nd | nd | 10.976 | nd | nd | nd | |
| TLB 2-9 | LC-MS/MS | na | na | 0.982 | 0.965 | 1.207 | na | na |
| Colorimetric method | 15.679 | 10.202 | 13.119 | nd | 12.762 | nd | nd | |
| TPA 1-3 | LC-MS/MS | na | na | 2.397 | 2.260 | 2.226 | na | na |
| Colorimetric method | 16.512 | 13.893 | 21.839 | nd | 18.506 | 12.792 | 15.351 | |
| TKC 2-1 | LC-MS/MS | na | na | na | na | 0.724 | 0.558 | 0.557 |
| Colorimetric method | nd | nd | nd | nd | nd | 31.869 | nd | |