Figure 4.

Sphaerodactylomelol effects on MCF-7 cells apoptosis biomarkers (A) externalization of phosphatidylserine, (B) Caspase-9 activity, and (C) DNA condensation and fragmentation. Values represent the mean ± SEM of three or four independent experiments. Symbols represent significant differences (p < 0.05) when compared to: * control. Images of 4′,6-diamidino-2-phenylindole (DAPI) stained cells were obtained using inverted fluorescence microscope at ×400. Arrows indicate alterations in DNA as comparing with control. The images are representative of one well of each situation tested.