Figure 1.

Effect of albumin depletion in the FBS-containing culture supernatant for proteome analysis. The undepleted and albumin-depleted samples were analyzed, with n = 3 each. (A) Amount of albumin by non-depletion and albumin depletion when normalized by the equivalent volume of the supernatant was analyzed by LC-MS. (B) Bar graph of the number of human and bovine proteins observed by proteome analyses. (C) Comparison of cellular localization by gene ontology (GO) enrichment analysis for the identified human proteins. (D) Venn diagram of the number of human proteins observed by proteome analyses. For (B,C), the results of the MS data individually analyzed by the Scaffold DIA software were used. For (D), the results of the simultaneously analyzed MS data collected for each treatment by the Scaffold DIA were used. Abbreviations: ALB, serum albumin.