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. 2021 Mar 4;22(5):2565. doi: 10.3390/ijms22052565

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Effect of the volume of the medium used on proteome analysis. (A) Bar graph of the number of human proteins observed in albumin-depleted supernatant of cultured in 10, 5, and 2 mL of medium containing 10% FBS. (B) Venn diagram of the number of human proteins observed in the three treated samples. For (A), the results of the MS data individually analyzed by the Scaffold DIA software were used. For (B), the results of the simultaneously analyzed MS data collected for each treatment by the Scaffold DIA were used. Abbreviations: ALB, serum albumin; Sup, supernatant.