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Immunocytochemistry of adherently grown cancer stem-like cell populations. (A) Representative immunostainings of co-expressed cancer stem cell (CSC) markers CD44/CD133 as well as Nestin, localized within the cytoplasm. (B) Exemplary depicted MYC expressions from one of each CSC population showed high frequencies of nuclear localizations of the analyzed protein. DAPI served in all cases for nuclear counterstaining.
