Table 3.
Results of oxidative hemolysis inhibition assay (OxHLIA), cytotoxic, hepatotoxic, and anti-inflammatory activity of the extracts from Impatiens walleriana (orange colour_WO and pink colour_WP) (mean ± SD).
| WO | WP | t-Students Test p-Value |
|
|---|---|---|---|
| Antioxidant activity (Ec50 values; µg/mL) | |||
| Oxidative hemolysis inhibition assay (OxHLIA) | 59 ± 7 | 34 ± 2 | <0.01 |
| Non-tumour cell lines (GI50 values; µg/mL) | |||
| PLP2 | >400 | >400 | <0.01 |
| Tumour cell lines (GI50 values; µg/mL) | |||
| HeLa (cervical carcinoma) | 177.3 ± 9.9 | 215.6 ± 8.8 | <0.01 |
| HepG2 (hepatocellular carcinoma) | 207.1 ± 16.6 | 277.9 ± 9.2 | <0.01 |
| MCF7 (breast adenocarcinoma) | 290.9 ± 9.4 | 307.72 ± 14.9 | <0.01 |
| NCI-H460 (non-small cell lung cancer) | 333.4 ± 10.5 | >400 | <0.01 |
| Anti-inflammatory (GI50 values; µg/mL) | |||
| Inhibition of NO production in LPS stimulated RAW 264.7 cells | 312.1 ± 5.5 | 349.21 ± 12.8 | <0.01 |
EC50 values: Extract concentration corresponding to 50% of the antioxidant activity. GI50 values: concentration that inhibits 50% of cell growth. Ellipticin GI50 values (positive control): 1.21 μg/mL (MCF-7), 1.03 μg/mL (NCI-H460), 0.91 μg/mL (HeLa), 1.10 μg/mL (HepG2), and 2.29 μg/mL (PLP2). GI50 > 400 μg/mL has no activity.