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. 2021 Mar 5;13(5):1107. doi: 10.3390/cancers13051107

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Hedgehog activation in NSCLC samples. (A) Immunohistochemistry analysis of Gli1 in NSCLC, patients X and Y are representative of Gli1+ patients (18/36). (B) mRNA expression of Gli1 target genes in NSCLC, data are expressed as mean ± SEM of 12 patients analyzed in duplicate. Two lung tissue samples not expressing Gli1 as assessed by IHC were used as reference samples or calibrators. A sample from basocellular carcinoma were used as positive control both for the IHC and the RT-qPCR. NSCLC: non-small-cell lung cancer.