Table 4.
Primers used for long-read sequencing.
| Patients | Age (year) | Interruptions | PCR Product (bp) | 5′ Modification | Buffer Sequence | Barcode Name | Barcode | Primer ST-barcoded-300-F | Primer ST-barcoded-300-R |
|---|---|---|---|---|---|---|---|---|---|
| E1 | 56 | Yes (CCG) | 2551 | Phosphate | GGTAG | BC1018_Forward | TCACGTGCTCACTGTG | GGTAGTCACGTGCTCACTGTGGAACTGTCTTCGACTCCGGG | GGTAGTCACGTGCTCACTGTGGCACTTTGCGAACCAACGAT |
| E2.1 | 29 | Yes (CCG) | 1864 | Phosphate | GGTAG | BC1019_Forward | ACACACTCTATCAGAT | GGTAGACACACTCTATCAGATGAACTGTCTTCGACTCCGGG | GGTAGACACACTCTATCAGATGCACTTTGCGAACCAACGAT |
| E3 | 0 | Yes (CCG) | 1150 | Phosphate | GGTAG | BC1020_Forward | CACGACACGACGATGT | GGTAGCACGACACGACGATGTGAACTGTCTTCGACTCCGGG | GGTAGCACGACACGACGATGTGCACTTTGCGAACCAACGAT |
| L2 | 31 | No | >5000 | Phosphate | GGTAG | BC1012_Forward | ACACTAGATCGCGTGT | GGTAGACACTAGATCGCGTGTGAACTGTCTTCGACTCCGGG | GGTAGACACTAGATCGCGTGTGCACTTTGCGAACCAACGAT |
| L3 | 26 | No | >5000 | Phosphate | GGTAG | BC1013_Forward | CTCTCGCATACGCGAG | GGTAGCTCTCGCATACGCGAGGAACTGTCTTCGACTCCGGG | GGTAGCTCTCGCATACGCGAGGCACTTTGCGAACCAACGAT |
| A4.1 | 32 | Yes (CAG) | 760 | Phosphate | GGTAG | BC1016_Forward | CATAGAGAGATAGTAT | GGTAGCATAGAGAGATAGTATGAACTGTCTTCGACTCCGGG | GGTAGCATAGAGAGATAGTATGCACTTTGCGAACCAACGAT |
| 1201 | 32 | No | 796 | Phosphate | GGTAG | BC1017_Forward | CACACGCGCGCTATAT | GGTAGCACACGCGCGCTATATGAACTGTCTTCGACTCCGGG | GGTAGCACACGCGCGCTATATGCACTTTGCGAACCAACGAT |
| B2 | 33 | Yes (CCG) | 1291 | Phosphate | GGTAG | BC1014_Forward | CTCACTACGCGCGCGT | GGTAGCTCACTACGCGCGCGTGAACTGTCTTCGACTCCGGG | GGTAGCTCACTACGCGCGCGTGCACTTTGCGAACCAACGAT |
| 5289 | 37 | No | 1141 | Phosphate | GGTAG | BC1015_Forward | CGCATGACACGTGTGT | GGTAGCGCATGACACGTGTGTGAACTGTCTTCGACTCCGGG | GGTAGCGCATGACACGTGTGTGCACTTTGCGAACCAACGAT |