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. 2021 Mar 6;13(5):1135. doi: 10.3390/cancers13051135

Figure 3.

Figure 3

Dinaciclib shows potent anti-proliferative activity in Ishikawa and HEC-1A cells, and is cytotoxic in Ishikawa cells. Total cell numbers were counted using an automated cell counter in (a) Ishikawa and (d) HEC-1A samples following treatment with DMSO, or 10 or 40 nM dinaciclib for 24, 48, or 72 h. Trypan Blue was used to identify dead cells within total cell counts as shown in (b) and (e) for Ishikawa and HEC-1A cells, respectively. Cell numbers are expressed as a percentage of control values. Apoptosis in (c) Ishikawa and (f) HEC-1A samples was measured using the RealTime-Glo Annexin V Apoptosis and Necrosis Assay following 12 and 24 h of treatment with DMSO, or 10, 40, or 80 nM of dinaciclib. Statistical significance was calculated by ANOVA followed by Dunnett’s test. * p value < 0.05, ** p value < 0.01, *** p value < 0.001, **** p value < 0.0001.