Figure 2.

Effect of BTK inhibitors on TPA-induced intracellular signaling pathways in MCF-7 cells. The cells were pretreated with BTK inhibitors for 1 h and then stimulated with TPA. Cell lysates were analyzed by western blotting using anti-p-PLCγ2 and anti-PLC γ2 antibodies (A). MCF-7 cells were pretreated with PLC inhibitors for 1 h and then stimulated with TPA. Cell lysates were subjected to SDS-PAGE and analyzed by western blotting using an anti-MMP-9 antibody (B). Data are the mean ± SEM of three independent experiments. ^#P<0.01 vs. untreated control; *P<0.01 vs. TPA. The blot was reprobed with an anti-β-actin antibody to confirm equal loading.