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. 2021 Mar 11;45(5):59. doi: 10.3892/or.2021.8010

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Copyright: © Liu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 1. — LncRNA GATA3-AS1 expression is upregulated in human PC tissues and cell lines. (A) The expression of GATA3-AS1 in 30 pairs of PC tissues and matched normal tissues was assessed by qPCR. *P<0.05, compared with the normal tissues. (B) The expression of GATA3-AS1 in normal human pancreatic duct epithelial cell line HPDE6-C7, and in PC cell lines BxPC-3, Capan-2, SW1990, PANC-1, and AsPC-1 was assessed by qPCR. *P<0.05, compared with the HPDE6-C7 group. (C and D) Two GATA3-AS1 siRNAs (si-GATA3-AS1-1 and si-GATA3-AS1-2) and negative control siRNA (siRNA NC) were transfected into PANC-1 or AsPC-1 cells, respectively. Then, the expression of GATA3-AS1 was detected by qPCR. *P<0.05, compared with the siRNA NC group. LncRNA, long noncoding RNA; GATA3-AS1, GATA3-antisense RNA 1; PC, pancreatic cancer; qPCR, quantitative polymerase chain reaction; siRNA or si-, small interfering RNA; NC, negative control.