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. 2021 Mar 11;45(5):59. doi: 10.3892/or.2021.8010

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Copyright: © Liu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — Knockdown of GATA3-AS1 affects the cell biology and stem cell-like characteristics of PC cells. (A and B) Cell Counting Kit-8 assay at 0, 24, 48, 72 and 96 h post transfection. (C) Cell proliferation of PANC-1 and AsPC-1 cells was assessed by colony formation assay. (D) Cell apoptosis of PANC-1 and AsPC-1 cells was assessed by flow cytometry. (E) Cell invasion of PANC-1 and AsPC-1 cells was assessed by Transwell assay. (F) The stemness of PANC-1 and AsPC-1 cells was assessed by spheroid formation assay. *P<0.05, compared with the siRNA NC group. (G) The PANC-1 or AsPC-1 cells infected with lentivirus-shRNA NC or lentivirus-sh-GATA3-AS1 were subcutaneously injected into the axilla of nude mice, respectively. Two weeks later, the primary tumors were weighed. *P<0.05, compared with the shRNA NC group. GATA3-AS1, GATA3-antisense RNA 1; PC, pancreatic cancer; siRNA or si-, small interfering RNA; shRNA or sh-, short hairpin RNA; NC, negative control.