Skip to main content
. 2021 Mar 7;22(5):2689. doi: 10.3390/ijms22052689

Figure 3.

Figure 3

ATP13A2 regulates αsyn membrane association and multimerization. (a) Analysis of αsyn protein levels by Western blot in the cytosolic and membrane fraction of control and ATP13A2 WT cells using β-catenin as a marker for the membrane fraction and tubulin as a marker for the cytosolic fraction. (b) Quantification of membrane-associated αsyn relative to β-catenin (n = 3). Statistical analysis was performed with column statistics (one-sample t-test) comparing test values to the hypothetical value of 1. The error bars represent the SEM. * p < 0.05. (c) Analysis of αsyn protein levels in the membrane fraction of ATP13A2 KD, control, ATP13A2 WT, and ATP13A2 DN cells without and with oxidative stress by Western blot using β-catenin as a standard. (d) Quantification of membrane-associated αsyn relative to β-catenin (n = 3). Statistical analysis was performed using a one-way ANOVA test with Bonferroni. The error bars represent the SEM. * p < 0.05, ns, not significant. (e) Cell lysates of cross-linked membrane fractions from ATP13A2 KD, control, ATP13A2 WT, and ATP13A2 DN cells without and with oxidative stress. (f) Quantification of ratio of membrane-associated αsyn multimers to DSP-αsyn. Statistical analysis was performed using a one-way ANOVA test with Bonferroni. The error bars represent the SEM. *** p < 0.001, ** p < 0.01, * p < 0.05, ns, not significant.