Figure 3.

DART helium gas temperature optimization in QqQ-MS/MS for DON (blue) and 3-AcDON (red) 60 ng/mL, in MeOH/NH₃ 2% v/v (solid line) and MeOH/NH₃ 2% v/v exposed to the ID-LFIA substrate (dotted line). Measurements were performed in duplicate by MRM monitoring of the ion transitions specified in Table 1. The standard deviation of the duplicate measurement is shown by the error bars. The results are presented as the relative (%) area intensity, i.e., the ratio of the absolute area intensity divided by the highest absolute area intensity observed for each substance in each solution.