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. 2021 Mar 7;21(5):1861. doi: 10.3390/s21051861

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(a). Representative chronograms for DON-spiked wheat samples used to develop the ID-LFIA, retrieved in dissociation solution and analyzed by DART-QqQ-MS/MS, demonstrating the three distinct spiking levels in blue 0.5× TL, orange 1× TL, and green 1.5× TL. ¹³C₁₅ DON is added as an internal standard before the MS/MS analysis. (b). Data analysis of 21 blank wheat samples, and spiked versions thereof in ID-LFIA/DART-QqQ-MS/MS, at three target level concentrations in blue 0.5× TL, orange 1× TL, and green 1.5× TL.