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. 2021 Mar 7;21(5):1861. doi: 10.3390/s21051861

Table 2.

Results from ID-LFIA/DART-QqQ-MS/MS analysis and the respective LFIA smartphone screening of 21 validation blank and spiked wheat samples at three different target levels and performed on three different days.

Day Sample 0× TL 0.5× TL 1× TL 1.5× TL
ID-LFIA/DART-QqQ-MS/MS
Response Factor Versus 13C15 DON ± SD		 LFIA Smartphone Screening Result (mg/kg, Mean Response ± SD) ID-LFIA/DART-QqQ-MS/MS LFIA Smartphone Screening Result (mg/kg, Mean Response ± SD) ID-LFIA/DART-QqQ-MS/MS LFIA Smartphone Screening Result (mg/kg, Mean Response ± SD) ID-LFIA/DART-QqQ-MS/MS LFIA Smartphone Screening Result (mg/kg, Mean Response ± SD)
Mean Ion Area Ratio of DON ± SD Expected mg/kg ± SD Mean Ion Area Ratio of DON ± SD Expected mg/kg ± SD Mean Ion Area Ratio of DON ± SD Expected mg/kg ± SD
1 1 0 <0.25 0.30 (±0.03) 0.80 (±0.01) 0.74 (±0.01) 0.27 (±0.03) 1.55 (±0.06) 1.74 (±0.01) 0.29 (±0.05) 2.40 (±0.02) 2.65 (±0.00)
2 0 <0.25 0.30 (±0.00) 0.73 (±0.01) 0.65 (±0.02) 0.32 (±0.01) 1.28 (±0.03) 1.30 (±0.01) 0.30 (±0.03) 2.77 (±0.01) 2.41 (±0.01)
3 0 <0.25 0.29 (±0.02) 0.97 (±0.06) 0.73 (±0.01) 0.28 (±0.01) 1.70 (±0.03) 1.72 (±0.02) 0.32 (±0.01) 2.99 (±0.03) 2.43 (±0.02)
4 0 <0.25 0.28 (±0.04) 0.98 (±0.01) 0.62 (±0.01) 0.34 (±0.00) 1.84 (±0.04) 1.76 (±0.01) 0.28 (±0.00) 2.91 (±0.11) 2.14 (±0.03)
5 0 <0.25 0.28 (±0.01) 0.83 (±0.03) 0.64 (±0.00) 0.30 (±0.02) 1.33 (±0.01) 1.86 (±0.02) 0.27 (±0.00) 2.91 (±0.12) 2.49 (±0.03)
6 0 <0.25 0.32 (±0.01) 0.86 (±0.02) 0.65 (±0.01) 0.23 (±0.00) 1.71 (±0.04) 1.85 (±0.03) 0.32 (±0.02) 2.42 (±0.00) 2.70 (±003)
7 0 <0.25 0.31 (±0.02) 0.68 (±0.02) 0.76 (±0.00) 0.31 (±0.02) 1.37 (±0.00) 1.79 (±0.02) 0.30 (±0.01) 2.58 (±0.06) 2.43 (±0.01)
2 8 0 <0.25 0.34 (±0.00) 0.84 (±0.02) 0.76 (±0.00) 0.31 (±0.02) 1.57 (±0.02) 1.28 (±0.01) 0.31 (±0.02) 2.98 (±0.01) 2.38 (±0.02)
9 0 <0.25 0.34 (±0.01) 0.75 (±0.02) 0.68 (±0.01) 0.25 (±0.00) 1.51 (±0.03) 1.18 (±0.01) 0.29 (±0.03) 2.53 (±0.01) 2.42 (±0.00)
10 0 <0.25 0.26 (±0.01) 0.71 (±0.03) 0.75 (±0.01) 0.27 (±0.02) 1.47 (±0.06) 1.36 (±0.01) 0.29 (±0.02) 2.87 (±0.07) 2.15 (±0.02)
11 0 <0.25 0.31 (±0.02) 1.06 (±0.01) 0.73 (±0.00) 0.28 (±0.05) 1.46 (±0.02) 1.38 (±0.00) 0.28 (±0.04) 2.82 (±0.00) 2.34 (±0.07)
12 0 <0.25 0.31 (±0.01) 0.69 (±0.00) 0.58 (±0.01) 0.28 (±0.01) 1.46 (±0.00) 1.34 (±0.01) 0.33 (±0.02) 2.81 (±0.01) 2.49 (±0.05)
13 0 <0.25 0.30 (±0.04) 0.80 (±0.01) 0.79 (±0.01) 0.30 (±0.02) 1.59 (±0.01) 1.14 (±0.01) 0.31 (±0.04) 2.90 (±0.05) 2.50 (±0.00)
14 0 <0.25 0.26 (±0.01) 0.88 (±0.00) 0.75 (±0.01) 0.24 (±0.00) 1.56 (±0.00) 1.58 (±0.03) 0.27 (±0.01) 2.73 (±0.00) 2.51 (±0.00)
3 15 0 <0.25 0.31 (±0.03) 0.92 (±0.02) 0.81 (±0.00) 0.26 (±0.03) 1.80 (±0.07) 1.95 (±0.02) 0.29 (±0.02) 2.65 (±0.01) 2.67 (±0.02)
16 0 <0.25 0.33 (±0.02) 0.99 (±0.03) 0.69 (±0.01) 0.25 (±0.02) 2.12 (±0.02) 1.76 (±0.02) 0.29 (±0.05) 2.73 (±0.03) 2.47 (±0.03)
17 0 <0.25 0.33 (±0.01) 0.94 (±0.01) 0.70 (±0.02) 0.31 (±0.03) 1.47 (±0.03) 1.37 (±0.01) 0.29 (±0.01) 2.69 (±0.04) 2.47 (±0.01)
18 0 <0.25 0.30 (±0.04) 0.97 (±0.03) 0.73 (±0.05) 0.25 (±0.00) 1.57 (±0.01) 1.64 (±0.02) 0.26 (±0.01) 2.84 (±0.07) 2.70 (±0.03)
19 0 <0.25 0.27 (±0.02) 1.03 (±0.01) 0.84 (±0.04) 0.30 (±0.03) 1.70 (±0.02) 1.80 (±0.01) 0.31 (±0.01) 2.92 (±0.07) 2.68 (±0.01)
20 0 <0.25 0.29 (±0.00) 0.79 (±0.02) 0.83 (±0.06) 0.26 (±0.02) 1.62 (±0.01) 1.33 (±0.01) 0.34 (±0.00) 2.64 (±0.01) 2.71 (±0.03)
21 0 <0.25 0.32 (±0.00) 0.79 (±0.03) 0.79 (±0.01) 0.25 (±0.00) 1.62 (±0.06) 1.69 (±0.05) 0.32 (±0.01) 2.69 (±0.05) 2.35 (±0.25)

Conditions: a duplicate sampling of the same final ID-LFIA dissociation solution in DART-QqQ-MS/MS. SD is the standard deviation of the duplicate measurements. The screening result was performed with the smartphone r-Biopharm LFIA, and the quantification of the screening was done with the associated smartphone app. SD is the standard deviation of duplicate reading of the same LFIA. The expected mg/kg is the concentration estimate calculated from each day’s calibration curve based on the response factor (mean ratio of DON MRM product ion at m/z 249.2 to the 13C15 DON MRM product ion at m/z 263.2). The DON ion ratio is the mean ratio of the absolute area of the DON MRM product ion at m/z 231.1 to the area of the ion at m/z 249.2.