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. Author manuscript; available in PMC: 2022 Jan 1.
Published in final edited form as: J Proteome Res. 2020 Nov 13;20(1):715–726. doi: 10.1021/acs.jproteome.0c00616

Figure 1: Design and workflow of Lck-TurboID.

Figure 1:

J.Lck.TurboID cells were generated by the transfection of pEF6-Lck-TurboID into Lck-deficient Jurkat E6–1 T cells made using CRISPR-Cas9. J.Lck.TurboID cells were grown in biotin-free RPMI with 2.5% regular:7.5% dialyzed serum for 2 weeks before 1 mM biotin supplementation ± TCR stimulation for 30 minutes. Cells were washed, lysed, enriched via streptavidin and processed for mass spectrometry as described.