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. Author manuscript; available in PMC: 2022 Jan 1.
Published in final edited form as: Br J Dermatol. 2020 Jun 24;184(1):96–110. doi: 10.1111/bjd.19115

Figure 4.

Figure 4

Effects of corticotropin-releasing hormone (CRH) and caffeine on the catagen-inducing factor transforming growth factor (TGF)-β2 in organ-cultured hair follicles. (a) Hair follicles were stained with immunofluorescence-labelled antibodies for TGF-β2 immunoreactivity (green fluorescence), which was detected in the outer root sheath (ORS) and partly in the inner root sheath (IRS). DP, dermal papilla. Bars = 50 μm. (b) Evaluation of the fluorescence intensity was conducted using Image J 1·38d software within the indicated white dashed rectangular areas. The data are expressed as the pooled mean ± SEM. *P < 0·05, ***P < 0·001.