Skip to main content
. Author manuscript; available in PMC: 2022 Jan 1.
Published in final edited form as: Br J Dermatol. 2020 Jun 24;184(1):96–110. doi: 10.1111/bjd.19115

Figure 5.

Figure 5

Effects of corticotropin-releasing hormone (CRH) and caffeine on the hypothalamic–pituitary–adrenal axis-dependent stress parameter CRH receptors (CRH1/2) in human hair follicles. (a) CRH-R1/2 immunoreactivity (green fluorescence) was detected in the outer root sheath (ORS) and inner root sheath (IRS) (white dashed rectangular areas), with membrane-bound and cytosolic detection (magnification in upper right image). Single fibroblasts in the dermal papilla (DP) were also CRH-R1/2 positive. CTS, connective tissue sheath. Bars = 50 μm. (b) Relative fluorescence intensity was measured with Image J 1·38d software within the dashed rectangular areas along the IRS and ORS. Data are presented as the mean ± SEM. **P < 0·01, ***P < 0·001.