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. Author manuscript; available in PMC: 2021 Mar 16.
Published in final edited form as: J Am Chem Soc. 2021 Feb 1;143(5):2293–2303. doi: 10.1021/jacs.0c10923

Figure 1.

Figure 1.

Yields of succinate, Fmoc-proline (P5C), and ethylene after incubation of 10 μM wt or D191E EFE with 40 μM (NH4)2Fe(SO4)2, 200 μM 2OG, and 500 μM of unlabeled L-arginine (L-Arg), perdeuterated L-arginine (d7-L-Arg), or 5,5-d2-L-arginine (d2-L-Arg) in air-saturated 40 mM sodium HEPES (pH 7.5) buffer at 21°C for ~10 minutes. 2OG was completely consumed during the reaction. P5C was derivatized to Fmoc-proline prior to quantification. Ethylene analysis was performed by GC-FID; succinate and Fmoc-proline were quantified by LC-MS. Error bars correspond to standard errors of the mean values for two experiments. Detailed procedures are provided in the Supporting Information.