Table 1.

Rate constants for formation and decay of the RO ferryl complex obtained by fitting the ΔA₃₁₈-versus-time data shown in Figure 2 to an A + B → C → D model using KinTek Explorer. Initial reactant and O₂ concentrations were reduced by a factor that reflects flux through the RO pathway (i.e., divided by 3.5 for wt and 1.05 for D191E). ε₃₁₈ and ε₅₁₀ values for the reactant complexes were ~ 40 and 90 M⁻¹ cm⁻¹, respectively, and ε₃₁₈ and ε₅₁₀ for the ferryl complexes were ~1450 and 190 M⁻¹ cm⁻¹, respectively.

	substrate	kformation, net (103 M−1 s−1)	kdecay (s−1)	kH/kD
wt	L-Arg	54	16
wt	d7-L-Arg	54	1	16
D191E	L-Arg	31	42
D191E	d7-L-Arg	31	1.6	26