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. 2021 Mar 16;10(5):e12076. doi: 10.1002/jev2.12076

FIGURE 1.

FIGURE 1

Strategy for enriching RNPs in EVs. [A. Com and CD63 fusion proteins. Com was fused to the N‐terminus, C‐terminus or both termini of CD63 with linker peptide in between. The sequence for linker 1 and linker 2 were ‘GGHNSGGGGGQSPGPAA’ and ‘SGGGGSMASNFTQFVLVDNGGTGDV’ respectively. B. Cartoon showing the recruitment of Cas9 or ABE RNPs into exosomes. RNPs associate with Com‐CD63‐Com on the plasma membrane through com/Com interaction and then enter the endosome system via endocytosis (step 1). The early endosomes become multivesicular bodies (MVB) following the inward budding of the outer endosomal membrane (step 2). The intraluminal vesicles are released into the medium as exosomes when the membranes of MVBs fuse with the cell membrane (step 3). Here Com is drawn as a monomer but may function as a homodimer. C. Cartoon showing the recruitment of Cas9 RNPs into microvesicles (step 4)]