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. Author manuscript; available in PMC: 2021 Nov 1.
Published in final edited form as: Addict Biol. 2019 Nov 16;25(6):e12843. doi: 10.1111/adb.12843

FIGURE 3.

FIGURE 3

LTD in control and reinstated animals depends on CB1R. A, (left) Average time course of synaptic response before and after LTD induction for slices from drug-naïve animals with and without Rimonabant (5 μM). Inset above: sample average pre- and post-traces for the two treatment groups. *P < .05, 2-way ANOVA, main effect of time and treatment groups. Note that these animals did not receive vehicle treatment but expressed similar LTD amplitudes as compared with the vehicle controls in Figure 1D, indicating that intensive handling of the rats is not necessary for LTD expression. (right) Bar graph summarizes the average response 15 to 25 minutes after LTD induction. MSNs showed robust LTD in untreated slices that was blocked by Rimonabant. *P < .05, comparing baseline response with response 15 to 25 postinduction. B, Bar graph summarizing changes of PP-ratio, sEPSP IEI, and amplitude 15 to 25 minutes after LTD induction. LTD in untreated slices was accompanied by a significant decrease in sEPSC amplitude (white). Incubation in Rimonabant decreased in sEPSC IEI (orange). *P < .05, comparing baseline response with response 15 to 25 postinduction. C, (left) Average time course of synaptic response before and after LTD induction for slices from animals that underwent cue-induced reinstatement with and without incubation in Rimonabant. *P < .05, 2-way ANOVA, main effect of time and treatment groups. Inset above: sample average pre- and post-traces for the two treatment groups. (right) Bar graph summarizing the average response 15 to 25 minutes after LTD induction. MSNs showed robust LTD in untreated slices but LTD was blocked by incubation with the CB1R antagonist Rimonabant. *P < .05, comparing baseline response with response 15 to 25 postinduction. D, Bar graph summarizing changes of PP-Ratio, sEPSP IEI, and amplitude 15 to 25 minutes after LTD induction. LTP in slices incubated with Rimonabant was accompanied by a significant decrease in PP-Ratio and sEPSC IEI (orange). *P < .05, comparing baseline response with response 15 to 25 postinduction N shown as cells/animals recorded