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. Author manuscript; available in PMC: 2021 Nov 1.
Published in final edited form as: Addict Biol. 2019 Nov 16;25(6):e12843. doi: 10.1111/adb.12843

FIGURE 4.

FIGURE 4

THC + CBD-induced metaplasticity can be reversed by CB1R PAM GAT-211. A, Average time course of CB1 agonist WIN55,212–2-dependent inhibition of fEPSPs in Ctrl, Ext, and Rst groups. *P < .05, 2-way ANOVA, main effect of time and treatment groups. B, Dose-response curves for WIN55,212–2. The inhibition induced by the 5-μM dose was significantly stronger for control as compared with Ext and RST rats. Four to seven slices were used to generate each point. *P < .05, multiple t tests with Holm-Sidak post hoc. C, GAT211, a positive allosteric Modulator of CB1, rescues LTD after THC + CBD self-administration. Green, open symbols represent average response for extinguished animals from Figure 1D as comparison. D, Bar graph illustrating change in PP-Ratio, sEPSC IEI, and amplitude as compared with baseline. Significant reduction in sEPSC amplitude indicates postsynaptic locus of LTD. #P = .029, Student’s t test. E, GAT211 had no effect on baseline synaptic transmission N = 12/4