Skip to main content

View full-text article in PMC

. 2021 Mar 7;22(5):2695. doi: 10.3390/ijms22052695

Table 2.

Primer sequences used during the quantitative RT-PCR (RT-qPCR).

Gene	Primer Sequence (5’ to 3’)		Annealing Temperature
csd1	Forward	TCGCATACACAGGGGTGTTA	54 °C
	Reverse	TGCGCCTTATCCCTAATGAC	54 °C
csd2	Forward	CCTTTCTTTGGTGGGTTTGA	54 °C
	Reverse	GCTCTTTATTGTGGGGCAAA	54 °C
csd3	Forward	CGCTCATTCAAGCCCTTATC	57 °C
	Reverse	GCTAAAAGGGGGTCATTGGT	57 °C
csd4	Forward	TTAAACCCACCAGGCTCATC	54 °C
	Reverse	GGCTTGTGTTCTTGGGTGTT	54 °C
csd6	Forward	GCAGAAATTAGAGCGCTTGG	51 °C
	Reverse	GCCCTTGGTGTTCAATTCAT	51 °C
amiA	Forward	ATACGGTTTGCTTTGGATGC	54 °C
	Reverse	GTCCGCAAAAATTACCCTGA	54 °C
ureA	Forward	TTTCACGCTAACGGCTTTTT	54 °C
	Reverse	AACCGGATGATGTGATGGAT	54 °C
glmM	Forward	CAACCGCTTGAGAAGAAAGG	54 °C
	Reverse	CCAACCAATTAAGCCAGGAA	54 °C
gyrB	Forward	CGTCGCTTTGGATCATTTTT	55 °C
	Reverse	AATGGCGTGCCACTTTTAAC	55 °C