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HPLC-measured concentration of L-glutamate (a), L-glutamine (b), L-aspartate (c) and L-asparagine (d) in the spent medium of wild-type P. aeruginosa PAO1 and the ΔaauR mutant at 6.0 and 9.0 h post inoculation. Data points represent mean values ± SD obtained from triplicate bacterial samples grown at 37 °C in glucose-minimal media supplemented with an initial concentration of 20 mM of indicated amino acid. ND denotes not detected
