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. 2021 Mar 16;14:54. doi: 10.1186/s13041-021-00767-w

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — Human TMEM132D interacts with Nap/Sra via C-terminal domains. a Exemplar Western blot showing CoIP of various human TMEM132D (V5-tagged) mutants and NAP1 (GFP-tagged). Expression was driven by constitutive CMV promoters in HEK293 cells and lysates were used for GFP-trap and blot with antibodies against GFP and V5. b Exemplar Western blot showing CoIP of various human TMEM132D (V5-tagged) mutants and SRA1 (GFP-tagged). 60 a.a. D, TMEM132D with deletion of the last 60 a.a. sequence. 120 a.a. D, TMEM132D with deletion of the last 120 a.a. sequence. WIRS, TMEM132D with the WIRS-like motif (KFTTFTAV) mutated to alanine residues. ECD, TMEM132D with only extracellular domain and transmembrane domain. Results represent three independently repeated experiments. c Schematic showing human TMEM132D or C. elegans TMEM-132 with various domain genetic deletions and a summary of their interaction with Nap homologs (human NAP1 and C. elegans GEX-3) from both CoIP and Y2H assays