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. 2021 Mar 16;12:183. doi: 10.1186/s13287-021-02247-3

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Analysis of pericyte biology in a mouse model of hindlimb ischemia. a Surgery to induce hindlimb ischemia was performed on the left leg of the mouse. After dissociating the artery from the vein, a segment of the artery was electrocauterized. b Schematic graph of the timeline for sample collection after ischemia surgery. IS, ischemia; Exp, experiment (i.e., flow cytometry and FACS). c The Doppler images show the blood flow in the ischemic and contralateral limbs at 0, 2, 4, and 7 days after surgery. The red signal represents the abundant blood flow. d The quantitation of blood flux of ischemic hindlimbs (left) relative to contralateral hindlimbs (right). The measurement was performed by MoorLDI software (version 5.2, Moore Instruments, Axminster, UK). Student’s t test was used to perform statistical analyses (*p < 0.05; **p < 0.01). IS, immediately after ischemia surgery. The mouse numbers used for Doppler scanning on ischemia day 0, 2, 4, and 7 were 4, 4, 3, and 5, respectively. e The strategy used to identify endothelial cell and pericyte populations in flow cytometry and FACS experiments