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. 2021 Mar 16;12:183. doi: 10.1186/s13287-021-02247-3

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Dynamics of endothelial cell and pericyte populations upon ischemia. a Representative images of endothelial cell (CD31⁺CD146⁻) and pericyte (CD146⁺CD31⁻) populations in gated CD45⁻ populations from ischemic gastrocnemius and soleus muscles. b Representative flow cytometry images of subtypes of pericytes based on Nestin expression. c Quantitation of the endothelial cell population in the CD45⁻ population from ischemic muscles. d Quantitation of the pericyte population in the CD45⁻ population from ischemic muscles. e The ratio of endothelial cell (EC) events/pericyte events. f, g The ratios of type I (f) and type II (g) pericytes in pericyte populations. Student’s t test was used to perform statistical analyses of the data presented in c–g. *p < 0.05; **p < 0.01. The numbers of mice used for flow cytometry on ischemia D0, D2, D4, and D7 were 6, 5, 6, and 6, respectively