Table 1.
MetAFLP quantitative characteristics of the regenerants derived via somatic embryogenesis using tissue culture conditions of the M1–M9 trials and ANOVA results, including Tukey’s HSD tests
| MetAFLP characteristics | Tissue culture conditions | |||||||
|---|---|---|---|---|---|---|---|---|
| TCIV | SV | DMV | DNMV | CuSO4 (µM) | AgNO3 (µM) | Length of induction step (days) | ||
| ANOVA | F | 32.676 | 14.612 | 12.177 | 7.274 | |||
| p | 0.0001 | 0.0001 | 0.0001 | 0.0001 | ||||
| Trials | M1 | 7.75c | 2.01bc | 1.92c | 1.63bc | 0.1 | 0 | 21 |
| M2 | 8.23c | 2.78bcd | 1.92c | 1.34c | 0.1 | 10 | 28 | |
| M3 | 7.94c | 2.21d | 1.92c | 1.53c | 0.1 | 60 | 35 | |
| M4 | 7.85c | 2.11d | 2.40b | 1.34c | 5 | 60 | 28 | |
| M5 | 7.66c | 2.11d | 2.40b | 1.34c | 5 | 0 | 35 | |
| M6 | 8.13c | 2.59 cd | 2.40b | 1.63bc | 5 | 10 | 21 | |
| M7 | 9.67b | 3.83a | 2.40b | 1.53c | 10 | 10 | 35 | |
| M8 | 10.67ab | 3.47ab | 2.41a | 2.22ab | 10 | 60 | 21 | |
| M9 | 10.77a | 3.37abc | 2.41a | 2.31a | 10 | 0 | 28 | |
The TCIV, SV, DMV, and DNMV reflect tissue culture-induced variation, sequence variation, demethylation, and de novo methylation, respectively. The a, b, and c indicate Tukey’s HSD grouping