Table 2.
MetAFLP characteristics | Tissue culture conditions | |||||||
---|---|---|---|---|---|---|---|---|
TCIV | SV | DMV | DNMV | CuSO4 (µM) | AgNO3 (µM) | Length of induction step (days) | ||
ANOVA | F | 9.117 | 0.192 | 16.997 | 7.009 | |||
p | 0.001 | 0.827 | 0.0001 | 0.004 | ||||
Trial | M10 | 12.76a | 4.34a | 4.41a | 1.26ab | 0.1 | 0 | 21 |
M12 | 11.01b | 4.22a | 3.65c | 0.72b | 10 | 60 | 21 | |
M13 | 13.66a | 4.10a | 3.98b | 2.26a | 2.95 | 15 | 28 |
M10 states for control, whereas M12-M13 for the verification trials. TCIV, SV, DMV, and DNMV, reflect tissue culture-induced variation, sequence variation, demethylation, and de novo methylation. The a, b, and c indicate Tukey’s HSD grouping. The presented data does not include outliers that were excluded based on the Grubs test