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. 2021 Mar 16;28:9. doi: 10.1186/s40709-021-00138-5

Table 2.

The arrangement of ANOVA statistics and Tukey’s HSD tests for trials in the verification process

MetAFLP characteristics Tissue culture conditions
TCIV SV DMV DNMV CuSO4 (µM) AgNO3 (µM) Length of induction step (days)
ANOVA F 9.117 0.192 16.997 7.009
p 0.001 0.827 0.0001 0.004
Trial M10 12.76a 4.34a 4.41a 1.26ab 0.1 0 21
M12 11.01b 4.22a 3.65c 0.72b 10 60 21
M13 13.66a 4.10a 3.98b 2.26a 2.95 15 28

M10 states for control, whereas M12-M13 for the verification trials. TCIV, SV, DMV, and DNMV, reflect tissue culture-induced variation, sequence variation, demethylation, and de novo methylation. The a, b, and c indicate Tukey’s HSD grouping. The presented data does not include outliers that were excluded based on the Grubs test