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. 2021 Mar 8;22(5):2712. doi: 10.3390/ijms22052712

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Inhibition of constitutive FGFR-1 ectodomain shedding by MMP inhibitors. COS 7 cells transiently transfected with FGFR-1(IIIc) were placed in serum-free media in the presence of different concentrations of (A) marimastat and (B) CGS 27023A, as indicated. The samples were run in duplicate. The conditioned media was collected after an overnight incubation and the shed FGFR-1 ectodomain was precipitated with WGA-Sepharose. The samples were analyzed by SDS-PAGE and immunoblotting with Mab6. The densities of the bands were measured with NIH Image software and the percent change in intensity of the bands was calculated using the control lane (0 uM) as a reference for comparison. Standard deviations were calculated for each individual band by the imaging software.