Figure 4.

Amplitudes of depolarizing spontaneous fluctuations (DSFs) are enhanced by in vivo cisplatin treatment and normalized by in vitro IL-10 incubation at depolarized membrane potentials. A. Average DSF amplitudes per neuron increased in cisplatin-treated mice at RMP and when neurons were artificially depolarized to −45 mV, (mice: PBS n=3 and cisplatin n=6) without being reversed by IL-10 incubation. Kruskal-Wallis test with Dunn’s correction for multiple comparisons p=0.0195, PBS vs. cisplatin: p=0.0155. B. Mean DSF amplitudes binned by RMP ranging from −65 and −41 mV from DRG neurons isolated from PBS and cisplatin treated mice exposed to vehicle or IL-10 (10 ng/ml) in vitro. f denotes statistical significance for cisplatin-treated neurons from the marked bin compared with the previous one. Data are shown as mean ± SEM.