Table 1.
Summary of C1A-VH3-53 antibody binding and neutralization data
| Antibody | Number of a.a. changes (VH, VL) | Kinetic analysis |
SARS-CoV-2 pseudotype |
SARS-CoV-2 WA1/2020 |
||
|---|---|---|---|---|---|---|
| ka (1/Ms) | kd (1/S) | KD (nM) | IC50 μg mL−1 | IC50 μg mL−1 | ||
| C1A-gl | – | 4.41E+04 | 5.59E−03 | 127 | 0.098a | 0.126 |
| C1A-gl∗ | – | 1.08E+05 | 4.95E−03 | 45.7 | 0.025a | 0.102 |
| C1A-B3 | 3, 2 | 1.04E+05 | 7.95E−03 | 76.3 | 0.053 | 0.441 |
| C1A-F10 | 1, 4 | 4.52E+04 | 2.51E−03 | 55.7 | 0.008 | 0.184 |
| C1A-C2 | 3, 6 | 1.70E+05 | 2.39E−03 | 14.1 | 0.118 | 0.132 |
| C1A-H5 | 5, 5 | 7.27E+04 | 6.14E−04 | 8.5 | 0.139 | 0.256 |
| C1A-C4 | 2, 5 | 5.23E+04 | 4.09E−04 | 7.8 | 0.046 | 0.127 |
| C1A-B12 | 1, 3 | 8.61E+04 | 3.63E−04 | 4.2 | 0.081 | 0.062 |
| C1A-H6 | 2, 4 | 1.13E+05 | 1.00E−04 | 0.9 | 0.072 | 0.112 |
| C1A-B12.1 | 3, 3 | 1.60E+05 | 1.07E−04 | 0.7 | 0.054a | 0.028 |
| C1A-B12.2 | 4, 3 | 1.69E+05 | 7.51E−05 | 0.5 | 0.115a | 0.025 |
| C1A-B12.3 | 5, 3 | 1.14E+05 | 6.23E−05 | 0.5 | 0.090a | 0.032 |
a.a., amino acids; ND, not determined.
a
Measured with D614GS lentivirus pseudotypes as reported in Figure 4.