Skip to main content
. 2021 Mar 16;184(9):2316–2331.e15. doi: 10.1016/j.cell.2021.03.029

Figure 2.

Figure 2

COV2-2676 and COV2-2489 binding map to the NTD of SARS-COV-2 S protein

(A) Top row (side view) and bottom row (top view) of Fab-S6Pecto closed trimer (S protein model PDB: 7JJI) complexes visualized by negative-stain electron microscopy for COV2-2676 Fab model in pink, COV2-2489 Fab model in blue, and superimpose 3D volume of CoV2-S-Fab 2676 complex in gray and CoV2-S-Fab 2489 in mesh. The S-NTD is shown in yellow and electron density in gray. Representative two-dimensional (2D) class averages for each complex are shown at the bottom (box size is 128 pixels, with 4.36 Å per pixel). Data are from a single experiment; detailed collection statistics are provided in Table S2.

(B) Identification of critical contact residues by alanine-scanning mutagenesis. Top (side view) with loss of binding residues (cyan) for COV2-2489 or COV2-2676 to mutant S-NTD constructs, normalized to the wild type. Bottom, escape mutations mapped to the NTD region for COV2-2489 (green G142D, R158S) or COV2-2676 (orange F140S).

(C) Results of viral selections with COV2-2489 or COV2-2676 individual mAbs. The number of replicates in which escape variants were selected is indicated. Mutations present in the NTD of the selected escape variants are indicated.