REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
COV2-2196	Zost et al., 2020a	N/A
COV2-2130	Zost et al., 2020a	N/A
COV2-2489	Zost et al., 2020a	N/A
COV2-2676	Zost et al., 2020a	N/A
COV2-2490	Zost et al., 2020a	N/A
COV2-2263	Zost et al., 2020a	N/A
CR3022	ter Meulen et al., 2006	N/A
r2D22	Smith et al., 2012	N/A
SARS2-38 (mouse monoclonal)	This paper	N/A
Goat anti-human lambda, mouse ads-UNLB	Southern Biotech	RRID: AB_2795760
Goat anti-human kappa, mouse ads-UNLB	Southern Biotech	RRID: AB_2795728
Goat anti-human IgG Fc, Multispecies ads-HRP	Southern Biotech	RRID: AB_2795580
Goat anti-human IgG-HRP	Southern Biotech	Cat# 2040-05, RRID: AB_2795644
Goat anti-human IgG Alexa Fluor 647	Invitrogen	Cat# A-21445
SARS2-16 (mouse monoclonal)	This paper	Michael S. Diamond laboratory, Washington University
Bacterial and Virus Strains
VSV-SARS-CoV-2	Case et al., 2020b	N/A
G142D VSV-SARS-CoV-2 (COV2-2489 escape mutant)	This paper	N/A
R158S VSV-SARS-CoV-2 (COV2-2489 escape mutant)	This paper	N/A
F140S VSV-SARS-CoV-2 (COV2-2676 escape mutant)
SARS-CoV-2 (strain 2019 n-CoV/USA_WA1/2020)	CDC/BEI Resources	NR52281
SARS-CoV-2 (isolate B.1.1.7)	Chen et al., 2021b	N/A
SARS-CoV-2 (B.1.351 spike protein chimeric virus)	Chen et al., 2021b	N/A
Chemicals, Peptides, and Recombinant Proteins
SARS-CoV-2 S6Pecto	Greaney et al., 2021	N/A
Avidin–Peroxidase	Sigma	Cat# A3151
1-step Ultra TMB-ELISA substrate solution	Thermo Fisher Scientific	Cat# 34029
ExpiCHO Expression Medium	Thermo Fisher Scientific	Cat# A2910001
FreeStyle 293 Expression Medium	Thermo Fisher Scientific	Cat# 1238002
Fetal Bovine Serum, ultra-low IgG	Thermo Fisher Scientific	Cat# 16250078
EZ-Link™ NHS-PEG4-Biotin, No-Weigh™ Format	Thermo Fisher Scientific	Cat# A39259
FabALACTICA® Fab kit	Genovis	Cat# A2-AFK-025
BioLock biotin blocking	IBA Lifescience	Cat# 2-0205-050
Uranyl Formate	EMS	Cat# CF400-CU-50
Critical Commercial Assays
MagMAX™-96 Viral RNA Isolation Kit	Thermo Fisher Scientific	AM1836
MagMAX™ mirVana™ Total RNA Isolation Kit	Thermo Fisher Scientific	A27828
TaqMan RNA-to-Ct 1-Step Kit	Thermo Fisher Scientific	4392939
Mouse Cytokine Array / Chemokine Array 31-Plex (MD31)	Eve Technologies	MD31
Deposited Data
COV2-2489 Fab complex with SARS-CoV-2 S6Pecto	This paper	EMDB: EMD-23154
COV2-2676 Fab complex with SARS-CoV-2 S6Pecto	This paper	EMDB: EMD-23155
Experimental Models: Cell Lines
Monkey: Vero E6	ATCC	ATCC: CRL-1586, RRID: CVCL_0574
Monkey: Vero E6 +TMPRSS2	Diamond laboratory, Washington University	N/A
Monkey: Vero E6 + ACE2 + TMPRS22	A. Creanga and B. Graham (Vaccine Research Center, NIH)	N/A
Monkey: Vero CCL-81	ATCC	CCL-81; RRID: CVCL_0059
Monkey: Vero Furin	Mukherjee et al., 2016	N/A
Monkey: MA104	ATCC	ATCC CRL-2378.1
Hamster: ExpiCHO-S	Thermo Fisher Scientific	Cat# A29127, RRID: CVCL_5J31
Human: FreeStyle 293-F	Thermo Fisher Scientific	Cat# R79007, RRID: CVCL_D603
Human: HEK293T	ATCC	ATCC CRL-3216
Human: HEK293T+ACE2	Diamond laboratory, Washington University	N/A
Human: HEK293	ATCC	ATCC CRL-1473
Experimental Models: Organisms/Strains
Mouse: B6.Cg-Tg(K18-ACE2)2Prlmn/J	Jackson Laboratory	Cat# 034860; RRID:IMSR_JAX:03486
Oligonucleotides
SARS-CoV-2 N F: 5′-ATGCTGCAATCGTGCTACAA-3′	Hassan et al., 2020	N/A
SARS-CoV-2 N R: 5′-GACTGCCGCCTCTGCTC-3′	Hassan et al., 2020	N/A
SARS-CoV-2 N Probe: 5′-/56-FAM/TCAAGGAAC/ZEN/AACATTGCCAA/3IABkFQ/-3′	Hassan et al., 2020	N/A
Gapdh TaqMan Primer/Probe set	IDT	Mm.PT.39a.1
CACACTTTCCTCGTGAAGG	This paper	VSV RNA amplification
CACTATTAAATTGGTTGGCAATC	This paper	VSV RNA amplification
GTACAATCACTTCTGGTTGG	This paper	VSV RNA amplification
GAACAGCAACCTGGTTAGAAG	This paper	VSV RNA amplification
CAGAGACATTGCTGACACTAC	This paper	VSV RNA amplification
GTCTACAGCATCTGTAATGG	This paper	VSV RNA amplification
GATTCTTCTTCAGGTTGGACAG	This paper	VSV RNA amplification
Forward-AGCTTCTGAACAATCCCCGG	This paper	VSV DNA Sequencing
Reverse-GAGGCCTCTTTGAGCATGGT	This paper	VSV DNA Sequencing
Recombinant DNA
Plasmid: pTwist-mCis_G1 COV2-2489	Zost et al., 2020a	N/A
Plasmid: pTwist-mCis_G1 COV2-2676	Zost et al., 2020a	N/A
Plasmid: pTwist-CMV S6Pecto	Greaney et al., 2021	N/A
Plasmid: pTwist-mCis_G1 rCR3022	Zost et al., 2020a	N/A
Plasmid: pTwist-mCis_G1 COV2-2196	Zost et al., 2020a	N/A
Plasmid: pTwist-mCis_G1 COV2-2130	Zost et al., 2020a	N/A
Plasmid: pTwist-mCis_G1 COV2-2263	Zost et al., 2020a	N/A
Plasmid: pTwist-mCis_G1 COV2-2490	Zost et al., 2020a	N/A
Software and Algorithms
RTCA version 2.1.0	Acea Biosciences, Inc	RTCA Software, RRID: SCR_014821
Serial EM 3.7	N/A	SerialEMRRID: SCR_017293
Topaz 0.2.3	Bepler et al., 2019, Bepler et al., 2020	Topaz
UCSF Chimera 1.14	Pettersen et al., 2004	UCSF Chimera, RRID: SCR_004097
Cryosparc 3.0.0	Punjani et al., 2017	RRID: SCR_016501
FlowJo	FlowJo, LLC	v10
GraphPad Prism	GraphPad	v 9.0.0
Nanozoomer Digital Pathology	Hamamatsu	v2
Other
xCELLigence RTCA MP analyzer	Acea Biosciences, Inc	N/A
xCELLigence E-Plate 96 PET cell culture plates	Acea Biosciences, Inc	Cat# 300601010
ÄKTA pure chromatography system	GE Healthcare Life Sciences	N/A
Biostack microplate stacker	BioTek	N/A
FEI TF20 electron microscope with Gatan US4000 4k × 4k CCD camera	TFS	N/A
StrepTrap HP column	GE Healthcare Life Sciences	Cat# 28-9075-48
400 mesh copper EM grids	EMS	Cat# 22451
EL406 washer dispenser	BioTek	N/A
HisTrap Excel column	GE Healthcare Life Sciences	Cat# 17-3712-06
HiTrap MabSelect™ SuRe 5 mL column	GE Healthcare Life Sciences	Cat# 29-0491-04