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. 2021 Mar 2;10:594141. doi: 10.3389/fonc.2020.594141

Figure 1.

Figure 1

Screen for agents with a selective cytotoxicity for highly metastatic prostate cancer cells. (A) Diagram illustrates the results of four independent and sequential screens for cytotoxic agents against metastatic prostate cancer cells. One thousand one hundred-twenty drugs were tested in at least two concentrations. (B) Representative dose response testing fenbendazole in prostate cancer and normal prostate cells for 48 h. Fenbendazole exhibited: 1) greater cell killing in highly metastatic PC-3MLN4 compared to less metastatic PC-3M cells at 10 μM, p < 0.01 (Screen I); 2) greater cell killing in both highly metastatic PC-3MLN4 and DU-145LN4 cells compared to the less metastatic counterparts, p < 0.01 for cell killing at 1 and 10 µM (Screen II); 3) a greater than 40% cell killing in AT6.1 cells, p < 0.001 for dose above 0.1 µM (Screen III); and 4) minimal cytotoxicity in normal prostate epithelial nBE cells, p > 0.05 (Screen IV). Data shown are mean ± 95% confidence interval, N=4 per group.