Figure 2.

In vivo screening of the five lead hits in survival assays using disseminated Dunning rat AT6.1 prostate carcinoma cells. To generate pre-existing micrometastatic lesions, AT6.1 Dunning rat prostate carcinoma cells were injected intravenously into nude mice five days prior to treatment. Fenbendazole (A), fluspirilene (B), suloctidil (C), clofazimine (D) and niclosamide (E) were given three times a week via intraperitoneal injection until signs of morbidity were observed. The drugs were solubilized in DMSO and diluted with saline prior to injection. Shown is Kaplan-Meier survivals curves (left) and mean survival (right) of mice treated with either vehicle (control) or agents. Data shown are mean ± 95% confidence interval, N=10 per group, *p < 0.05.