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. Author manuscript; available in PMC: 2021 Mar 16.
Published in final edited form as: Brain Struct Funct. 2021 Jan 2;226(2):481–499. doi: 10.1007/s00429-020-02194-4

Fig. 5. 3D visualization of COLM acquired dual HCR labeling in iDISCO+ tissue.

Fig. 5.

(a) Dorsal view of a representative mouse hemisphere showing Pvalb (green, AF-647) and Vglut1 (red, AF-594) labeling in down-sampled image volume (b) Volume rendered view showing cellular resolution in ventral hippocampus and cortex (parts of lateral entorhinal cortex) in an extracted sub-volume. (c-d) Dorsal views of a different z-plane than (a) showing Pvalb and Vglut1 FISH signal. Insets, zoomed-in view. Images are pseudocolored for the overlaying purpose. Scale bars (μm) - (a) 1500; (b) 300; (c-d) 1000 (Inset-200).