KEY RESOURCES TABLE

REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit anti GFP (made in goat)	Novus	NB600-308, RRID: AB_1048879
Biotinylated anti rabbit IgG (made in goat)	Vector	BA-1000, RRID: AB_2336201
Goat anti rabbit conjugated with Alexa 488	Invitrogen	A-11034, RRID: AB_221544
DAB tetrahydrochloride	Sigma	D5637-5G
Cytochrome C	Sigma	C-2506
Bacterial and viral strains
Pseudorabies-GFP (strain 152)	Enquist lab
Lentivirus-CAG-synaptophysin-eGFP	Adrian Lozada
Retrograde lentivirus-hsyn-Cit	Daniel Gibbs
AAV2.5-hsyn-flex-Cit	John Lin (Lin et al., 2013)
AAV2.5-hsyn-flex-ReaChR-Cit	Salk Virus Core	Special preparation
AAV retro-hsyn-Cre	UPenn Virus Core	Special preparation
AAV-DJ-hsyn-flex-mGFP-2a-mRuby	Stanford Virus Core	GVVC-AAV-100
Retrograde lentivirus-hsyn-Cre	Fan Wang
Biological samples
Chemicals, peptides, and recombinant proteins
Critical commercial assays
Deposited data
Raw data	This paper
Experimental models: Cell lines
Experimental models: Organisms/strains
Mouse: C57BL/6J	Jackson Laboratories	#000664, RRID: MGI:5657312
Thy1-ChR2-YFP alias B6.Cg-Tg(Thy1-COP4/EYFP)18Gfng/J	Jackson Laboratories (Arenkiel et al., 2007; Wang et al., 2007)	#007612, RRID: IMSR_JAX:007615
Oligonucleotides
Recombinant DNA
Software and algorithms
MATLAB	Mathworks	2007b to 2017b
Chronux	Chronux.org	http://chronux.org
FIJI	NIH	http://imagej.net/Fiji