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. 2020 Dec 21;104(3):602–610. doi: 10.1093/biolre/ioaa227

Figure 6.

Figure 6

Dose–response effect of insulin-like growth factor 2 (IGF2) on proliferation and gene expression of cultured granulosa cells (GCs). (A) GC proliferation was assessed by cell counting. (B and C) GC follicle-stimulating hormone receptor (FSHR) and luteinizing hormone/choriogonadotropin receptor (LHCGR) mRNA levels were determined by real-time PCR. Mitochondrial ribosomal protein S10 (MRPS10) served as the internal control. *, significant difference between culture groups, P < 0.05. Data from three animals are presented as the mean ± SEM. One-way ANOVA followed by the Student–Newman–Keuls post hoc test was performed to compare GC proliferation and gene expression between culture groups.