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. 2021 Mar 2;11:614458. doi: 10.3389/fonc.2021.614458

Figure 3.

Figure 3

Effects of chidamide (CHI) and/or doxorubicin (DOX) on apoptosis of multidrug-resistant (MDR) breast cancer cells. (A) After treatment with CHI and/or DOX (48h), flow cytometry was used to detect apoptosis. Annexin V/PI staining was measured with flow cytometry. Representative plots of three independent experiments are shown. Quantitative values showed the average percentage of Annexin V-positive cells (lower right quadrant, both in early apoptosis; upper right quadrant, late apoptosis) of three independent experiments. (B) Apoptosis was determined using TUNEL staining assay. The number of TUNEL-positive cells (red) and DAPI-positive cells (blue) was visually measured. All samples were subjected to at least two biological replicate analyses, and three images of each replicate were obtained using a 20× objective to count TUNEL-positive cells and DAPI-positive cells. The percentage of TUNEL-positive cells was calculated as (TUNEL-positive cells/total cells) × 100. The numerical values are expressed as mean ± S (D) of three independent replicates. “*” indicates a significant difference compared with the control group (*P < 0.05, **P < 0.01),”#” indicates a significant difference compared with the DOX-treated group (##P < 0.01), and “&” indicates a significant difference compared with the CHI-treated group (&P < 0.05).