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. 2021 Mar 2;11:614458. doi: 10.3389/fonc.2021.614458

Figure 4.

Figure 4

Chidamide (CHI) combined with doxorubicin (DOX) induced cytotoxicity by driving p53/p21 to induce cell cycle arrest and caspase-dependent apoptosis. Cells were treated with DOX and/or CHI for 48 h. (A) The western blot analysis showed that CHI combined with DOX treatment down-regulated Bcl-xl, Bcl-2, caspase-9, caspase-7, caspase-3 and up-regulated p53, p21, Puma, Bax, cleaved caspase-9, cleaved caspase-7, cleaved caspase-3 in CALDOX and MCF-7/A02 cells. GAPDH was used as a loading control. (B) Fold changes in Bcl-2, Bax and caspase-3 mRNA levels were detected using RT-qPCR in MDR cells. The numerical values are expressed as mean ± SD of three independent replicates. “*” indicates a significant difference compared with the control group (*P < 0.05, **P < 0.01), “#” indicates a significant difference compared with the DOX-treated group (#P < 0.05, ##P < 0.01), and “&” indicates a significant difference compared with the CHI-treated group (&P < 0.05).