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. 2018 Apr 6;10(6):503–514. doi: 10.1093/jmcb/mjy024

Figure 2.

Figure 2

Effect of Emc10 disruption on sperm function. (A) The percentage of motile spermatozoa from wild-type (Wt) and Emc10-null (Ko) mice during 2-h incubation in medium (n = 4). (B−D) Comparison of path velocity (B), linear velocity (C), and track velocity (D) of spermatozoa from Wt and Ko mice (n = 4). (E and F) Measurement of capacitation-associated protein tyrosine phosphorylation (E) and PKA substrate phosphorylation (F) of spermatozoa from Wt and Ko mice. Spermatozoa were incubated in capacitating medium and collected for western blot per hour. α-tubulin served as the loading control. The western blot is representative of four independent experiments. (G) The percentage of uncapacitated (F pattern), capacitated (B pattern), and acrosome-reacted (AR pattern) spermatozoa from Wt and Ko mice. (H) Examination of the acrosome reaction induced by A23187 or progesterone in sperm from Wt and Ko mice (n = 7). Data are presented as mean ± SEM. ***P < 0.001 when compared with respective controls.