Skip to main content
. 2021 Mar 16;28(6):591–603. doi: 10.3727/096504020X15982623243955

Figure 2.

Figure 2

WEE2-AS1 functions as a molecular sponge for miR-520f-3p in GBM cells. (A) lncLocator was used to predict the subcellular localization of WEE2-AS1. (B) The subcellular distribution of WEE2-AS1 in T98 and U251 cells was tested by the subcellular fractionation assay. (C) The binding site between WEE2-AS1 and miR-520f-3p was predicted by StarBase version 3.0. The mutant sequences are shown. (D) Luciferase activity was detected in T98 and U251 cells that were cotransfected with miR-520f-3p mimic or NC mimic and WEE2-AS1-wild type (wt) or WEE2-AS1-mutant (mut). (E) RNA immunoprecipitation (RIP) assay was carried out in T98 and U251 cells, and the relative RNA level of WEE2-AS1 and miR-520f-3p in the immunoprecipitates was analyzed by qRT-PCR. (F) qRT-PCR was applied to measure miR-520f-3p expression in T98 and U251 cells after si-WEE2-AS1 or si-NC transfection. Transfection of si-WEE2-AS1 increased miR-520f-3p expression in T98 and U251 cells. (G) The miR-520f-3p expression in 59 GBM tissues and 19 normal brain tissues was examined by qRT-PCR. (H) Pearson’s correlation coefficient showed a negative correlation between miR-520f-3p and WEE2-AS1 expression in the 59 GBM tissues (r = −0.6719, p < 0.0001). **p < 0.01.