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. 2021 Mar 16;28(6):591–603. doi: 10.3727/096504020X15982623243955

Figure 4.

Figure 4

WEE2-AS1 positively regulates SP1 expression in GBM cells via sponging miR-520f-3p. (A, B) The mRNA and protein levels of SP1 in T98 and U251 cells were measured by qRT-PCR and Western blotting after the transfection of si-WEE2-AS1 or si-NC. (C) Pearson’s correlation coefficient was determined to assess the correlation between WEE2-AS1 and SP1 mRNA expression in the 59 GBM tissues (r = 0.6259, p < 0.0001). (D) qRT-PCR was conducted to detect miR-520f-3p expression in T98 and U251 cells after miR-520f-3p inhibitor or NC inhibitor transfection. (E, F) si-WEE2-AS1 was cotransfected with miR-520f-3p inhibitor or NC inhibitor into T98 and U251 cells. The cotransfected cells were collected and subjected to the measurement of SP1 mRNA and protein expression via qRT-PCR and Western blotting. **p < 0.01.