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. 2021 Mar 8;22(5):2733. doi: 10.3390/ijms22052733

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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L. gibba G3 DWC114 seed development and germination (A) Developing seed growing in the meristematic pouch, (B) Detached fruit with 2 developing seed. Note the two hyperpigmented spots, which are the opercula (white arrow). The operculum is visible on the free seed, which shows the typical ribbing on the seed coat. (C) Germinating seed showing emergence of the new green frond together with ungerminated seed at the bottom of the culture vessel. (D) A seedling floating on the surface of the medium still attached to the seed coat (white arrow), and already undergoing asexual budding. (E) Germination percent of seed over eight days in E in multi-well plates in LD or CL lighting conditions. Data are the average ± SEM (n = 3).