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. 2021 Mar 8;22(5):2729. doi: 10.3390/ijms22052729

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Endothelial NOX5 expression increased Cav1 expression in mesenteric and epididymal fat in mice fed a HFD for 10 weeks. (A,B) Cav1 mRNA levels in (A) mesenteric fat and (B) epididymal fat. (C,D) Representative Western blot and protein levels of CAV1 in total lysates of (C) mesenteric fat and (D) epididymal fat. Control diet: control Cre (n = 12), Nox5/Cre (n = 11); high-fat diet: control Cre (n = 10), Nox5/Cre (n = 12). Values are expressed as median with confidence interval. mRNA levels are relative to Gapdh. Protein levels are relative to B-ACTIN. ## p < 0.01: diet differences (dotted lines); * p < 0.05: genotype differences (solid lines). Statistical test used: Aligned Rank ANOVA.