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. 2021 Mar 8;22(5):2729. doi: 10.3390/ijms22052729

Figure 7.

Figure 7

Glut4 and Cav-1 expression was increased in 3T3-L1 adipocytes when incubated for 24 h with Glu + PA conditioned media from NOX5-expressing endothelial bEnd.3 cells. (A,C,E) Glut4, Ir-B, and Cav1 mRNA levels of 3T3-L1 adipocytes (n = 3). (B,D,F) GLUT4, IR-B, and CAV1 protein levels of 3T3-L1 adipocytes (n = 3). (G) Representative images of Western blot for GLUT4, IR-B, and CAV1. mRNA levels are relative to GAPDH; protein levels are relative to β-actin. Fold increase is relative to the control group (Nox(−)/Glu + PA(−)). Values are expressed as mean ± SEM; * p < 0.05, ** p < 0.01, *** p < 0.001: differences relative to Nox(−)/Glu + PA(−); # p < 0.05, ### p < 0.001: differences relative to Nox(+)/Glu + PA(-); $ p < 0.05, $$$ p < 0.001: differences relative to Nox(−)/Glu + PA(+). Statistical test used: ANOVA.