Figure 2.

Real time monitoring of breast cancer MCF-7 cell viability after 24 h exposure to synthetic compounds 1, 2, 3, 4, 5a, 5b, 5c, 5d, 5e, 5f, 5g, and 5h and 0.5% dimethyl sulfoxide (DMSO) vehicle (control), using the xCELLigence System Real-Time Cell Analyzer. (A) Normalized Cell Index (NCI) variations of MCF-7 cancer cells after 24 h incubation with 0.5% DMSO vehicle (Ctr, control) and 50 µM of cisplatin (CDDP), 1, 2, 3, 4, 5a, 5b, 5c, 5d, 5e, 5f, 5g, and 5h. NCI values are relative to controls treated with 0.5% DMSO vehicle. Data are presented as mean ± SD; n = 3. Statistical significances are referred to the DMSO control. Differences between groups were determined by analysis of variance (ANOVA) and Dunnett’s test was used for multiple comparisons with the control. * p < 0.05; *** p < 0.0001 (B) Left panel: Representative NCI traces of MCF-7 cells exposed to increasing concentrations (12.5, 25, 50 µM) of 5h and 0.5% DMSO vehicle (control) for 48 h. Right panel: Half-maximal inhibitory concentration (IC₅₀) values (±standard error, SE) of 5a, 5e, 5g, and 5h against MCF-7 cells after 24 h drug exposure. The IC₅₀ value represents the concentration of each compound that reduces the NCI by 50%. Data are means of three independent experiments.